Composite

Part:BBa_K4160012:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


GEMS receptor construct containing PR3 fused to HA-tag as affinity domain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2366
    Illegal PstI site found at 191
    Illegal PstI site found at 1054
    Illegal PstI site found at 2049
    Illegal PstI site found at 2210
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 760
    Illegal NheI site found at 1423
    Illegal PstI site found at 191
    Illegal PstI site found at 1054
    Illegal PstI site found at 2049
    Illegal PstI site found at 2210
    Illegal NotI site found at 2353
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 760
    Illegal BglII site found at 1527
    Illegal BglII site found at 1713
    Illegal BglII site found at 1977
    Illegal BamHI site found at 64
    Illegal XhoI site found at 937
    Illegal XhoI site found at 2360
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2366
    Illegal PstI site found at 191
    Illegal PstI site found at 1054
    Illegal PstI site found at 2049
    Illegal PstI site found at 2210
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 760
    Illegal XbaI site found at 2366
    Illegal PstI site found at 191
    Illegal PstI site found at 1054
    Illegal PstI site found at 2049
    Illegal PstI site found at 2210
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

This composite part combines BBa_K4160000, BBa_K4160001, BBa_K4160002, BBa_K4160004, BBa_K1150016, and BBa_K2217015.

References